General Information

  • ID:  hor001214
  • Uniprot ID:  Q9N4V0
  • Protein name:  FLP-18
  • Gene name:  flp-18
  • Organism:  Caenorhabditis elegans
  • Family:  FARP (FMRFamide related peptide) family
  • Source:  animal
  • Expression:  Expressed in head neurons and weakly in ventral nerve cord .
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Caenorhabditis (genus), Peloderinae (subfamily), Rhabditidae (family), Rhabditoidea (superfamily), Rhabditomorpha (infraorder), Rhabditina (suborder), Rhabditida (order), Chromadorea (class), Nematoda (phylum), Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0003674 molecular_function; GO:0031841 neuropeptide Y receptor binding
  • GO BP:  GO:0006937 regulation of muscle contraction; GO:0007218 neuropeptide signaling pathway; GO:0030431 sleep
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space

Sequence Information

  • Sequence:  EMPGVLRFG
  • Length:  9
  • Propeptide:  MQRWSGVLLISLCCLLRGALAYTEPIYEIVEEDIPAEDIEVTRTNEKQDGRVFSKRDFDGAMPGVLRFGKRGGVWEKRESSVQKKEMPGVLRFGKRAYFDEKKSVPGVLRFGKRSYFDEKKSVPGVLRFGKRDVPMDKREIPGVLRFGKRDYMADSFDKRSEVPGVLRFGKRDVPGVLRFGKRSDLEEHYAGVLLKKSVPGVLRFGRK
  • Signal peptide:  MQRWSGVLLISLCCLLRGALA
  • Modification:  T8 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  FMRFamide-like neuropeptides (PubMed:14555955, PubMed:23658528). Ligand to G-protein coupled receptor npr-1 (PubMed:14555955). Involved in modulating locomotion quiescence during the sleep-like state called lethargus which occurs during molting between larval and adult stages, acting via npr-1 (PubMed:23764289). Together with flp-1, plays a homeostatic role by acting on the GABAergic neural transmission at neuromuscular junctions to prevent overexcitation of the locomotor circuit (PubMed:23658528). Plays a role in the navigational capacity of sperm and the targeting of sperm derived from males to the fertilization site in the uterus of hermaphrodites (PubMed:28662030).
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q9N4V0-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor001214_AF2.pdbhor001214_ESM.pdb

Physical Information

Mass: 114807 Formula: C45H72N12O12S
Absent amino acids: ACDHIKNQSTWY Common amino acids: G
pI: 6.41 Basic residues: 1
Polar residues: 2 Hydrophobic residues: 3
Hydrophobicity: 25.56 Boman Index: -556
Half-Life: 1 hour Half-Life Yeast: 30 min
Half-Life E.Coli: >10 hour Aliphatic Index 75.56
Instability Index: 7911.11 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  17564681
  • Title:  Impaired processing of FLP and NLP peptides in carboxypeptidase E (EGL-21)-deficient Caenorhabditis elegans as analyzed by mass spectrometry.